The power of flow cytometry
- source: [ @rieseberg2001 Flow cytometry in biotechnology ]
- tags: #single-cell , #biomarkers
The power of flow cytometry resides, first, in the wide range of cellular parameters that can be analyzed at the same time. Secondly, on the single-cell nature of the method. Flow cytometry allows not only to detect markers in average, but as a distribution, which gives access to sub-population information.
To gain access to the relevant information, several parameters can be retrieved:
- Absorption (in the sense of forward-scatter)
- Scattering (in the sense of side-scatter)
- Fluorescence
The fact that flow cytometers are commercially, robust, and versatile, are what makes them successful in clinical and biotechnological applications.
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